Figure 6.

Inhibition of p38 blocks the suppressive role of sh-EZH2 in the proliferation and invasiveness of trophoblast cell lines. A, protein level and phosphorylation of p38 in HTR-8/SVneo cells after SB203580 treatment determined by WB analysis; B, viability of cells examined by the MTT assay; C, proliferation activity cells examined by the EdU labeling assay; D, migration ability of HTR-8/SVneo and JEG3 cells examined by the scratch test; E, invasiveness of HTR-8/SVneo and JEG3 cells examined by the transwell assay. Repetition = 3. *p < 0.05; **p < 0.01.