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. 2022 May 20;13(5):12309–12325. doi: 10.1080/21655979.2022.2072619

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© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — miR-744-5p downregulated MAPK signaling pathway through inhibiting TGFB1 expression. (a) TGFB1 was successfully transfected into 143B and MG-63 cell lines. (b-f) Colony formation and EdU assays demonstrated that miR-744-5p suppressed the proliferation of OS cells, and overexpression of TGFB1 could reverse the effect. (g-j) Transwell migration and invasion assays indicated that overexpressed miR-744-5p significantly suppressed the migratory and invasive ability of OS cells, and overexpressed TGFB1 could abolish the influence. (k-n) Western blotting assays showed that miR-744-5p downregulated metastasis-related, MAPK-related and TGFB1 proteins in OS cells, while TGFB1 own contrary functions. * p < 0.05, ** p < 0.01, *** p < 0.001.