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. 2022 May 29;13(5):13188–13200. doi: 10.1080/21655979.2022.2074770

Figure 5.

Figure 5.

miR-1323 targets the 3′-UTR of Nrf2. (a) Graphical depiction of the conserved miR-1323 binding motif at the Nrf2 3′-UTR. (b) DLRA was conducted using the luciferase reporter constructs, which contained either the Mut or WT of Nrf2 after miR-1323/NC mimic transfection. The firefly luciferase activity was standardized to Renilla luciferase activity. (c) Chondrocytes were exposed to pcDNA3.1-empty (NC) or pcDNA3.1-ZFAS1 (ZFAS1) transfection for one day and then treated with 0.5 μM LPS for another day. qRT-PCR was conducted to assess the Nrf2 mRNA level. (d) Chondrocytes were exposed to pcDNA3.1-ZFAS1 (ZFAS1), NC/miR-1323 mimic co-transfection for one day and then treated with 0.5 μM LPS for another day. qRT-PCR was conducted to evaluate the level of Nrf2 mRNA. LPS-treated chondrocytes vs. indicated or control or LPS+OE-ZFAS1+ NC mimic group (* P < 0.05, ** P < 0.01); LPS-treated chondrocytes vs. LPS+OE-ZFAS1 + 1323 mimic group ($$ P < 0.01).