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. Author manuscript; available in PMC: 2022 Jul 12.
Published in final edited form as: Dev Biol. 2017 May 13;427(1):12–20. doi: 10.1016/j.ydbio.2017.05.012

Fig. 5. RNA-seq of miR-205−/− tissues uncovers relevant miR-205 targets and pathways.

Fig. 5.

(A) Schematic overview of experimental method for tissue isolation and RNA sequencing. (B) Overlap between up-regulated genes and predicted miR-205 targets expressed in the surface ectoderm. (C) Luciferase UTR assays for subset of genes up-regulated in miR-205 knockout samples (SEM error bars). No binding site (NS), a perfect binding site (PS) control. (D) Luciferase UTR assays for miR-205 targets with wildtype UTRs (normalized to one) and mutated UTRs.