TABLE 1.
Bacterial strains, phages, and plasmids
| Strains, phages, and plasmids | Relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| SURE | Host for plasmids derived from pBC KS(+) and pBluescript SK(−); dam+ | Stratagene |
| HB101 | Propagation strain for pBC KS(+) plasmid; dam+ | Stratagene |
| KW251 | Propagation strain for λ recombinant bacteriophages; dam+ | Stratagene |
| VEC6831 | Host for plasmids derived from pG+Host9; dam+ | Provided by E. Maguin |
| S. thermophilus | ||
| A054 | Strain closely related to CNRZ368 which does not possess ICESt1 | Industrial |
| CNRZ368 | Strain closely related to A054 possessing ICESt1 | CNRZa |
| NST1010 | Derivative of CNRZ368 with sth368IR gene disrupted by pNST154 integration | This work |
| NST1013A | Derivative of A054 containing a copy of pNST153IS integrated into IS1195L | This work |
| L. lactis MG1363 | Plasmid free; transformation host | 14 |
| Phagesb | ||
| λNST101 | λ recombinant phage containing the left end of ICESt1 | 6 |
| λNST106 | λ recombinant phage containing an internal fragment of ICESt1 | 6 |
| λNST107 | λ recombinant phage containing an internal fragment of ICESt1 | 6 |
| λNST108 | λ recombinant phage containing an internal fragment of ICESt1 | 6 |
| λNST113 | λ recombinant phage containing the right end of ICESt1 | 6 |
| φST84 | Pac site lytic group II bacteriophage of S. thermophilus | 5 |
| Plasmidsb | ||
| pBC KS(+) | Cloning vector; Camr; 3.4 kb | Stratagene |
| pBluescript SK(−) | Cloning vector; Ampr; 2.96 kb | Stratagene |
| pG+Host9 | Thermosensitive shuttle vector; Eryr; 3.8 kb | 23 |
| pNST132.2 | 2.2-kb EcoRI/EcoRV fragment of λNST101 (1132.2) cloned into pBluescript SK(−) | This work |
| pNST141 | 3.3-kb XbaI fragment of λNST107 (I141) cloned into pBC KS(+) | This work |
| pNST142 | 2.2-kb XbaI fragment of λNST107 (I142) cloned into pBC KS(+) | This work |
| pNST144 | 5.1-kb EcoRI fragment of λNST107 (I144) cloned into pBC KS(+) | This work |
| pNST144.1 | 674-bp XmnI/ClaI fragment of pNST144 (I144.1) cloned into EcoRV/ClaI-digested pBC KS(+) | This work |
| pNST153 | 3.8-kb HindIII/SalI fragment of λNST106 (HS38) cloned into pG+Host9 | This work |
| pNST153IS | 3.8-kb HindIII/SalI fragment of λNST106 (HS38) and 0,9-kb HindIII fragment of pNST132.2 (H09) cloned into pG+Host9 | This work |
| pNST154 | EcoRI fragment of pNST144.1 cloned into pG+Host9 | This work |
| pNST155 | 75-bp Sau3AI fragment of pBC KS(+) cloned into BamHI-digested pBC KS(+) | This work |
| pNST156 | 1104-bp Sau3AI fragment of pBC KS(+) cloned into BamHI-digested pBC KS(+) | This work |
a
CNRZ, Centre National de la Recherche Zootechnique.
b
Locations of the chromosomal fragments on the map of ICESt1 are indicated in Fig. 1. Ampr, ampicillin resistance; Camr, chloramphenicol resistance; Eryr, erythromycin resistance.