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. 2022 Feb 15;135(8):920–929. doi: 10.1097/CM9.0000000000002037

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The characteristics of hnRNPA1-knockdown CLL cells. (A) The transcript level of hnRNPA1 in hnRNPA1-knockdown MEC-1 and JVM-3 was verified by qRT-PCR. (B) The protein level of hnRNPA1 in hnRNPA1-knockdown MEC-1 and JVM-3 was verified by Western blot. (C) The proliferative ability of hnRNPA1-knockdown MEC-1 as well as JVM-3 and corresponding negative control tested by CCK-8 assay. (D) The apoptosis of hnRNPA1-knockdown MEC-1 as well as JVM-3 and corresponding negative control detected by Annexin V-APC/7-AAD apoptosis kit. Data are representative of three independent experiments. ^∗P < 0.05. CCK-8: Cell count kit-8; CLL: Chronic lymphocytic leukemia; hnRNPA1: Heterogeneous nuclear ribonucleoprotein A1; qRT-PCR: Quantitative real-time polymerase chain reaction.