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. 2022 Jun 23;24(7):1129–1140. doi: 10.1038/s41556-022-00940-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — a) Scatter plots comparing miRNA RPMs in wild-type (X-axis) and mutants (Y-axis) as indicated under control RNAi conditions. Linear regression is used to fit the data. miRNA counts in each condition are averaged across two replicates. b) Scatter plots comparing sRNA RPMs in the F1 progeny of control and mex-6 RNAi fed P0 worms for the indicated genotypes. Each dot corresponds to a locus in the C. elegans genome. The red dot corresponds to the mex-6 locus. Linear regression is calculated without mex-6 sRNA counts. sRNA counts in each condition were averaged across two replicates. c) Linear regression statistics modelling the relationship between the two sRNAseq replicates for each genotype in both control and mex-6 RNAi conditions. d) Super plot showing data from Fig. 2d with nuclei colour-coded to indicate worm origin. Central black dot and error bars represent the mean and standard deviation respectively. The distribution of values from each worm overlap. See legend for Fig. 2d for further description.