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. 2022 Jun 27;23(7):1076–1085. doi: 10.1038/s41590-022-01230-1

Fig. 5. Plasmablasts in the steady state contain unique and recurrent clonotypes.

Fig. 5

a,b, Venn diagram depicts PB families shared between samples from D1 and D2 collected at the indicated time points. c,d, Honeycomb plots compile recurrent PB sequences from samples of different time points from D1 and D2 into clonal families (left) and corresponding isotype expressed by each cell in the family (right). The bar plots below show the percent total and recurrent PB families with a given isotype. e, PBs were isolated from D1 and D2 7 d after influenza vaccination in 2010–11 and plated on mesenchymal stromal cells. The culture supernatants were tested for the presence of IgG antibodies to influenza vaccine and to recall antigens to which the individual was not recently exposed. TT, tetanus toxoid; MV, measles virus, VZV, varicella zoster virus; TG, Toxoplasma gondii. f, The same analysis was performed on PBs isolated in the steady state. g, The specificity of the antibodies produced by single PBs of D1 was confirmed through the isolation of monoclonal antibodies against TT or MV.