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. 2022 Jun 27;23(7):1076–1085. doi: 10.1038/s41590-022-01230-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, The table shows the number of single memory B cells (MBCs) and plasmablasts (PBs) profiled from different time points from D1 and D2. b, Representative flow cytometry plots showing gating strategy for MBC and PB populations. c, Size distribution of MBC clonal families in D1 and D2 comprising one or more isotypes. Red lines show median values. d, Class-switch propensity matrices show the number of clonal families having clones of each possible upstream and downstream isotype pair. The number on the diagonal shows the families having a single isotype (not colored). e,f, Representative genealogic trees depicting the class-switching in clonal families from D1 and D2. The number of somatic mutations at nucleotide and at amino acid (in parenthesis) levels are indicated on individual branches of the trees. The vertical thin dotted lines without arrowheads connect cells with identical VH/VL sequences. The thickness of the arrows reflects the number of mutations. The order of isotypes in the branches of the family trees does not represent class-switching sequence. A comprehensive set can be found in the RepSeq Playground.