Fig. 4.

SIRT1 inhibition abolishes eNOS dimerization and endothelial vasodilation evoked by RSV in Mthfr^+/– mice. A Schematic representation of in vivo animal treatment. B Dose–response curves to acetylcholine (ACh) of mesenteric arteries from wild-type and Mthfr^+/– mice collected at the end of in vivo treatment of Group 1. C, D Dose–response curves to acetylcholine (ACh) of mesenteric arteries obtained from C wild-type and D Mthfr^+/– mice collected at the end of in vivo treatment of Group 2, in presence of SIRT1 inhibitor, EX527. E Measurement of SIRT1 activity in mice mesenteric arteries collected at the end of in vivo treatment. F Representative western blot analysis performed on mesenteric arteries extracted at the end of in vivo treatment. *p < 0.05; **p < 0.001. Statistical analysis was determined by two-way ANOVA followed by Bonferroni’s post hoc test