Figure 4.

CD34⁺CD19⁺ CLL cells from unmutated CLL patients expand and generate CLL cells in culture. Sorted CD34⁺CD19⁺ cells from 6 patients with unmutated CLL and cytogenetic abnormalities detectable by FISH were placed in liquid culture with 10ug/ml IL-2 and IL-3. The purity of the isolated CD34⁺CD19⁺ CLL cells was ≥96% for all 6 patients. The total cell expansion is shown in first column, and total FISH + cell expansion based on the % FISH + cells after culture (66.7 ± 8% of the cells harbored the CLL-specific chromosomal abnormality) is shown in the second column.