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. 2022 Jul 12;8:81. doi: 10.1038/s41523-022-00444-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Feature plots showing highest expression of KRT15, CD55, and ANXA1 (annexin A1) in the area of cluster 1.2 and highest expression of KIT, SLC34A2, and PODXL in the area of cluster 1.4. b Representative fluorescence multicolor imaging of cryostat sections of normal breast stained for CD55 or annexin A1 (green) and K15 (red), and nuclei (blue). Arrows indicate CD55⁺/K15⁺ or annexin A1⁺/K15⁺ luminal cells. Images with higher magnification of selected areas (white squares) are shown below. Note that annexin A1 also stains myoepithelial cells. Annexin A1 and CD55 staining colocalizes with K15 preferentially in ductal luminal cells. Scale bars = 50 μm.