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. 2022 Mar 26;127(1):69–78. doi: 10.1038/s41416-022-01783-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a MSCs were co-cultured with melanoma cells in transwells for 24 h. RNA from MSCs was isolated and analysed by qPCR. b MSCs were cultured for 48 h with control RPMI media and conditioned media (CM) from SKMEL28, A375, and specimen M#2. The DNA from MSCs were isolated and analysed by Taqman qPCR. c MSCs were cultured for 48 h with control RPMI media and CM. MSCs were stained with MitoTracker Green and analysed via flow cytometry. d MSCs were cultured for 48 h with control RPMI media and CM. MSCs were stained with TMRM and analysed via flow cytometry. e, f MSCs were cultured for 48 h with control RPMI media and CM. Seahorse extracellular flux analysis of the MSCs determined the influence of increased mitochondria on preferred mechanism of metabolism. n = 4, *p < 0.05. g, h MSCs were cultured for 48 h with control RPMI media and CM. Glucose consumption of MSC media and Glucose uptake by MSCs was determined. Data expressed as detected relative light units (RLU). n = 4, *p < 0.05.