Table 2.
Primers in experiments in HeLa cells.
| Primer name | Primer sequence(5’-3’) | Tm | Product size |
|---|---|---|---|
| β-actin | F:CATGGAGTCCTGTGGCATC | 59 °C | 157 bp |
| R:CAGGGCAGTGATCTCCTTCT | |||
| ACSL4 | F:AATGCAGCCAAATGGAAAAG | 60 °C | 152 bp |
| R:CACAGAAGATGGCAATGGTG | |||
| GPX4 | F:CAGTGAGGCAAGACCGAAGT | 59 °C | 111 bp |
| R:CTGCTTCCCGAACTGGTTAC | |||
| NCOA4 | F:GCACTTGATGGCTCATGCTA | 60 °C | 151 bp |
| R:ATAACCACTGGCAGGTTTGC | |||
| Fth1 | F:TGACAAAAATGACCCCCATT | 59 °C | 160 bp |
| R:CAGGGTGTGCTTGTCAAAGA | |||
| TFRC | F:AAAATCCGGTGTAGGCACAG | 59 °C | 179 bp |
| R:TTAAATGCAGGGACGAAAGG | |||
| SP1 | F:TCATACCAGGTGCAAACCAA | 60 °C | 224 bp |
| R:GCTGGGAGTCAAGGTAGCTG | |||
| MAVS | F:CCTAAGGCCCTCTCTTTGCT | 59 °C | 185 bp |
| R:GCACCTCCAAAGAGCTTGAC | |||
| TFAP2A | F:ACTGAGACTCCCGTCAATGG | 60 °C | 231 bp |
| R:GCGTGTTCCTTAATCCGTGT | |||
| STAT3 | F:CTGGCCTTTGGTGTTGAAAT | 59 °C | 202 bp |
| R:AAGGCACCCACAGAAACAAC | |||
| TFRCp-BS1 | F:GAGCCCAGGAGTTCAAGACTA | 58 °C | 150 bp |
| R:ATTCCTGACCTCAGGTGATCT | |||
| TFRCp-BS2 | F:TACGTGCCTCAGGAAGTGAC | 5 °C | 184 bp |
| R:AGTGGCAGAAACAGTGGATG | |||
| TFRCp-BS3 | F:GTACGTGCCTCAGGAAGTGA | 58°C | 150 bp |
| R:GAAATGACAACGAGGGGATG |
The primer of gene’s sequence, annealing temperature, and product size in PCR in HeLa cells.