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. 2022 Jul 11;13(7):594. doi: 10.1038/s41419-022-05051-w

Fig. 2. PCB2 alleviates I/R- or H/R-induced pulmonary cell apoptosis.

Fig. 2

a–f A549 cells were treated with 20 μM PCB2 for 24 h and then subjected to 6 h of hypoxia followed by 2 h of reoxygenation to achieve H/R injury. a Cell viability, n = 6. b Representative images of TUNEL staining in A549 cells; the nuclei were stained with DAPI. Scale bar = 200 μm. c TUNEL scores. The relative number of apoptotic cells is presented as TUNEL-positive cells/DAPI (n = 6). d Representative FACS dot plots of annexin V and propidium iodide dual labeling. e FACS analysis of the apoptotic rate in A549 cells. The sum of the annexin V-positive population and propidium iodide-positive population is shown. n = 3. f Caspase-3 activity in living A549 cells. n = 6. g–k C57BL/6 mice were subjected to 1 h of ischemia followed by 2 h of reperfusion to establish lung I/R injury; mice from the control group underwent sham surgical procedures. PCB2 (30 mg/kg) was injected intraperitoneally for three consecutive days before the operation. g Caspase-3 activity in the lung, n = 6. h, i Expression of the Bcl-2 protein in the lung, n = 3. j Representative images of TUNEL staining in the lung. Scale bar = 100 μm. k Apoptotic index of the lung, n = 3. *p < 0.05. Error bars depict the standard deviations.