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. 2022 Mar 5;127(1):30–42. doi: 10.1038/s41416-022-01757-y

Fig. 3. METTL14 inhibits ccRCC cell proliferation and migration in vitro.

Fig. 3

a CCK-8 assay was performed to measure the proliferation of Caki-1 and Caki-2 cells overexpressed or knockdown of METTL14. b EdU staining revealed the proliferation of Caki-1 and Caki-2 cells overexpressed or knockdown of METTL14. Bar = 100 μm. c Representative images and quantification of colony-formation assay of ccRCC cells overexpressed or knockdown of METTL14 in a 6-well plate (9.6 cm2). Magnification, ×200. d Transwell invasion assay was performed to determine the effects of METTL14 on the migration ability of ccRCC cells. Magnification, ×200. Bar = 100 μm. n = 6. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the indicated group. NC OE overexpression plasmid of negative control pcDNA3.1, METTL14 OE overexpression plasmid of METTL14, NC KD shRNA targeting negative control, METTL14 KD shRNA targeting METTL14.