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. 2022 Mar 5;127(1):30–42. doi: 10.1038/s41416-022-01757-y

Fig. 6. METTL14 overexpression enhances Pten mRNA stability via an m6A-YTHDF1-dependent manner.

Fig. 6

a, b m6A level of Caki-1 and Caki-2 cells overexpressed or knocked down of METTL14 was detected. c Effect of METTL14 on Pten mRNA m6A level in Caki-1 cells was measured by MeRIP. d RIP assay followed by qRT-PCR to assay Pten mRNA endogenously associated with YTHDF1 in Caki-1 cells. e YTHDF1 and Pten protein expression in Caki-1 cells overexpressed with YTHDF1 were detected by western blot. f After actinomycin D (5 mg/mL) treatment, RNA stability assay showed the mRNA half-life (t1/2) of Caki-1 cells overexpressed with YTHDF1. n = 6. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the indicated group. NC OE overexpression plasmid of negative control pcDNA3.1, METTL14 OE overexpression plasmid of METTL14, NC KD shRNA targeting negative control, METTL14 KD shRNA targeting METTL14, YTHDF1 OE overexpression plasmid of YTHDF1.