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. 2022 Mar 5;127(1):30–42. doi: 10.1038/s41416-022-01757-y

Fig. 7. METTL14 inhibits ccRCC cell proliferation, migration and the AKT signalling activation via an m6A-YTHDF1-dependent manner.

Fig. 7

METTL14 was overexpressed in Caki-1 cells with or without YTHDF1 knockdown. a qRT-PCR assay showed the mRNA expression of Pten in Caki-1 cells of the indicated group. b Western blot showed the expression of Pten in Caki-1 cells of the indicated group. c CCK-8 assay was performed to measure the proliferation of Caki-1 cells of the indicated group. d Representative images and quantification of colony-formation assay of Caki-1 cells of the indicated group. Magnification, ×200. e Transwell invasion assay was performed to determine the effects of YTHDF1 on METTL14’s regulation on migration ability of Caki-1 cells. Magnification, ×200. Bar = 100 μm. f PI3K, AKT, mTOR, S6K, 4EBP1 and the related phosphorylation protein level in Caki-1 cells were detected by western blot. n = 6. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the indicated group. NC overexpression plasmid of negative control pcDNA3.1+shRNA targeting negative control, METTL14 OE overexpression plasmid of METTL14 + shRNA targeting negative control, METTL14 OE + YTHDF1 KD overexpression plasmid of METTL14 + shRNA targeting YTHDF1.