Caki-1 cells with or without METTL14 knockdown were subcutaneously injected into each flank of the mice to establish the ccRCC xenograft model. Pten overexpression lentivirus was administrated by intratumoral injection after the tumour cells injection to overexpression Pten in the tumour tissues. a Pictures of the isolated tumours of the indicated group. b Total m6A level of tumours in the indicated group. c H&E and IHC staining of METTL14 and Pten of the tumour tissues. Bar = 400 μm. d PI3K, AKT, mTOR, S6K, 4EBP1 and the related phosphorylation protein level in tumour tissues were detected by using western blot. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, compared to the indicated group. NC overexpression plasmid of negative control pcDNA3.1+shRNA targeting negative control, METTL14 KD shRNA targeting METTL14 + overexpression plasmid of negative control pcDNA3.1, METTL14 KD + Pten OE shRNA targeting METTL14 + overexpression plasmid of Pten.