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. 2022 Jul 11;13(7):597. doi: 10.1038/s41419-022-05040-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Silver staining displayed proteins pulled down by SNORA50C and its antisense RNA. B, C RNA pull-down and RIP assays detected the binding of HDAC1 and SNORA50C in SK-N-AS and SK-N-SH cells. (Student’s t-test) ^**P < 0.01. Error bars indicate mean ± SD. (N = 3). D Western blot analyzed the level of HDAC1 protein in SK-N-AS and SK-N-SH cells with SNORA50C silence or overexpression. E, F Western blot showed the expression of HDAC1 protein in SNORA50C-depleted SK-N-AS and SK-N-SH cells with or without the protein synthesis inhibitor CHX or the proteasome inhibitor MG132. G, H Western blot detected HDAC1 levels in SK-N-AS and SK-N-SH cells transfected with Ctrl ASO or SNORA50C ASO#1 followed by CHX treatment at the indicated time points (left panel). Quantification of western blot results (right panel). (Student’s t-test) ^**P < 0.01. Error bars indicate mean ± SD. (N = 3). I SK-N-AS and SK-N-SH cells were transfected with SNORA50C ASO#1 and treated with MG132, and then cells were subjected to western blot analysis using anti-HDAC1 or anti-ubiquitin antibody.