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. 2022 Jul 11;12:11790. doi: 10.1038/s41598-022-15999-6

Figure 4.

Figure 4

Endogenous T2R38 ligand activity in skin extract and cell culture supernatants from HaCaT cells but not from fibroblasts. (A–C) Migration of T2R38 transduced Jurkat cells to recombinant human CXC chemokine ligand 12 (CXCL12) and the indicated amounts of diluted skin extract (A), HaCaT cell culture supernatants (B), or fibroblast cell culture supernatants (C). (D) Migration of empty vector-transduced Jurkat cells. Skin extract and cell culture supernatants were mixed with CXCL12 (100 ng/ml). The white bars indicate untransduced cells, and black bars indicate transduced cells. One representative of three independent experiments is shown. (A–C) n = 3; (D) n = 2. ***P < 0.001, **P < 0.01 by Student’s t-test.