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. 2022 Jul 12;12:11798. doi: 10.1038/s41598-022-16180-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bilirubin photoisomer calibration curves. (a) Fluorescence assay. Calibration curve was prepared from the fluorescence intensity of reference ZZ-BR adjusted to concentrations in the range of 0–5 μmol/L along with UnaG mixture (see Methods in the text). (b) Liquid chromatography–mass spectrometry. ZZ-BR and LR are expressed as the ratio of ion intensity to that of the internal standard, MBR. ZZ-BR, ZZ-bilirubin; LR, lumirubin; MBR, mesobilirubin.