TABLE 4.
Antimicrobial resistance profiles and MICs for donor, recipient, and transconjugant isolates
| Bacterial strain(s) | MICa (μg/ml) of:
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| AMC | AMP | CEF | CHL | KAN | NAL | STR | SUL | TET | |
| Donors | |||||||||
| CVM990 (E. coli O157:H7) | 4 | 4 | 4 | ≤4 | >64 | ≤4 | >256 | >512 | 32 |
| CVM1884 (E. coli O111:NM) | 4 | 4 | 8 | ≤4 | ≤16 | ≤4 | <32 | >512 | >32 |
| Recipients | |||||||||
| JM263 (E. coli O157:H7) | 4 | 4 | 32 | 32 | ≤16 | >256 | ≤32 | ≤128 | 8 |
| CVM1202 (H. alvei) | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | ≤32 | ≤128 | ≤4 |
| CVM1203 (H. alvei) | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | ≤32 | ≤128 | ≤4 |
| CVM1208 (H. alvei) | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | ≤32 | ≤128 | ≤4 |
| Transconjugants | |||||||||
| CVM990-JM263 | 4 | 8 | 32 | 32 | ≤16 | >256 | >256 | >512 | >32 |
| CVM990-CVM1202 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
| CVM990-CVM1203 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
| CVM990-CVM1208 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
| CVM1884-JM263 | 4 | 8 | 32 | 32 | ≤16 | >256 | ≤32 | >512 | >32 |
| CVM1884-CVM1202 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
| CVM1884-CVM1203 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
| CVM1884-CVM1208 | >32 | >32 | >32 | ≤4 | ≤16 | ≤4 | 128 | >512 | >32 |
a
MICs were determined via microdilution methods according to NCCLS standards (20, 21). Abbreviations: AMC, amoxicillin-clavulanic acid; AMP, ampicillin; CEF, cephalothin; CHL, chloramphenicol; KAN, kanamycin; STR, streptomycin; SUL, sulfamethoxazole; TET, tetracycline. Numbers in boldface represent resistant phenotypes.