Salicylate disrupts low-frequency hearing but not ultrahigh-frequency hearing. A Pure-tone mABR thresholds change after 200 mg/kg salicylate i.p. injection in 1-month-old C57BL/6 mice. Thresholds before the injection, just after the injection (0 min), and at 70 min, 110 min, one day (day 2) post-injection are shown. Thresholds at 12–20 kHz show significant elevation after injection and stabilize at 70 min post-injection. This elevation recovered on the second day. In contrast, 54–80 kHz thresholds remained unchanged. Before vs 110 min, Dunnett test, 12 kHz, **P = 0.0041; 16 kHz, ***P = 0.0005; 20 kHz, ****P <0.0001; 32 kHz, ****P <0.0001; 40 kHz, ****P <0.0001; 54 kHz, P = 0.1380; 63 kHz, P >0.9999; 80 kHz, P = 0.8995. Before vs day2, Dunnett test, 12 kHz, P = 0.8995; 16 kHz, P = 0.4577; 20 kHz, P >0.9999; 32 kHz, *P = 0.0280; 40 kHz, ***P = 0.0005; 54 kHz, P = 0.1380; 63 kHz, P = 0.4577; 80 kHz, P = 0.8995. B Time-lapse mABR threshold variation after salicylate injection. The injection time was defined as 0 min (gray arrow). Data are presented as the mean ± SD. n = 3.