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. 2022 Jun 29;13:854202. doi: 10.3389/fimmu.2022.854202

Figure 3.

Figure 3

Chronic stress induced extracellular histones release and extracellular trap formation in mPFC. (A) Representative immunofluorescence images showing the citH3+ and NeuN+ in CONT, CUMS, and CORT groups were presented, and citH3-ir neurons in mPFC was analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, ***P < 0.001, compared with CONT. Two replica for n = 3). Scale bars: 20× and, 40×. (B) Representative immunofluorescence images showing the relationship of F4/80+ microglia and citH3+ cells in CONT, CUMS, and CORT groups were presented. Scale bar: 126×. (C) Representative immunofluorescence images showing the relationship of citH3 and PAD4 in CONT, CUMS, and CORT groups were presented. Scale bar: 126×. (D) Representative Western blots showing extracellular histone H3 in CSF. (E) Concentration of nucleosomes in CSF was analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, ***P < 0.001, compared with CONT; n = 6). (F) Regression analysis between nucleosomes in CSF and IL-1β in mPFC was presented (linear regression; n = 6). (G) Representative Western blots showing citH3 and PAD4 in mPFC were presented. Relative expression of citH3 and PAD4 was analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, **P < 0.01, compared with CONT; n = 3). post hoc.