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. 2022 Jun 29;13:854202. doi: 10.3389/fimmu.2022.854202

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Copyright © 2022 Wu, Bao, Liu, Zhang, Huang, Quan, Li, Xiong, Chen and Hou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mice with chronic stress were accompanied with microglial activation in mPFC. (A) Representative immunofluorescence images showing the Iba-1⁺ microglia in CONT, CUMS, and CORT groups were presented, and the microglial density, area of cell body, and the MFI of Iba-1 were analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, ***P < 0.001, compared with CONT. Two replica for n = 6). Scale bar: 40×. (B) Representative immunofluorescence images showing the 3D reconstruction of Iba-1⁺ microglia in CONT, CUMS, and CORT groups were presented, and Sholl analysis and their AUCs were analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, ***P < 0.001, compared with CONT. Two replica for n = 6). Scale bar: 126×. (C) Representative immunofluorescence images showing the co-localization of CD68⁺ and Iba-1⁺ microglia in CONT, CUMS, and CORT group were presented, and the Iba-1⁺CD68⁺ microglial density and also the MFI of CD68 were analyzed (two-way ANOVA with Tukey’s post hoc testpost hoc, ***P < 0.001, compared with CONT. Two replica for n = 6). Scale bar: 63×.