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. 2022 Nov 1;42(6):638–649. doi: 10.3343/alm.2022.42.6.638

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© Korean Society for Laboratory Medicine

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2 — NK cells cultured in different culture media exhibit different receptor expression levels. (A) Histogram of cytotoxic effector molecule and surface receptor expression of UCB NK cells cultured RC (orange), DS (blue), or AI (red). The geometric means are indicated in the respective histograms. (B) Percent expression levels of cytotoxic effector molecules and surface receptors on day 28 (N=9). (C) Geometric mean expression levels of cytotoxic effector molecules and surface receptors on day 28 (N=9) (*P<0.05; **P<0.01; ***P<0.001).

Abbreviations: RC, RPMI medium+FBS; DS, DMEM+Ham’s F12+various supplements; AI, AIM V+ICSR; UCB, umbilical cord blood; NK, natural killer; RPMI, Roswell Park Memorial Institute; FBS, fetal bovine serum; DMEM, Dulbecco’s modified Eagle’s medium, ICSR, Immune Cell Serum Replacement.