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. 2022 Nov 1;42(6):638–649. doi: 10.3343/alm.2022.42.6.638

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© Korean Society for Laboratory Medicine

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Culture medium shifting is advantageous for restoring NK cell function. (A) Fold expansion of UCB NK cells cultured in DS (blue), DS-AI (purple), AI-DS (green), or AI (red) (N=8) on day 28. (B) Purity (CD56+/CD3− NK cells) of media-shifted UCB-expanded NK cells cultured in DS (blue), DS-AI (purple), AI-DS (green), or AI (red) (N=11) on day 28. Flow cytometry-based cytotoxicity assay of UCB-expanded NK cells cultured in DS, AI, AI-DS, or DS-AI at the indicated ratios; (C) K562 cells, N=7; (D) Raji cells, N=4; (E) Raji cells + rituximab, N=7 (C–E). ^{*, †}P<0.05; ^{**, ††}P<0.01; ^{***, †††}P<0.001; *DS vs. AI; ^†DS vs. DS-AI; ^‡AI vs. AI-DS.

Abbreviations: RC, RPMI medium+FBS; DS, DMEM+Ham’s F12+various supplements; AI, AIM V+ICSR; DS-AI, DS shifted to AI on day 14; AI-DS, AI shifted to DS on day 14; UCB, umbilical cord blood; NK, natural killer; RPMI, Roswell Park Memorial Institute; FBS, fetal bovine serum; DMEM, Dulbecco’s modified Eagle’s medium, ICSR, Immune Cell Serum Replacement.