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. 2022 Nov 1;42(6):638–649. doi: 10.3343/alm.2022.42.6.638

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© Korean Society for Laboratory Medicine

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5 — Killing behaviors of NK cells cultured in different media. (A) Representative time-lapse images of NK cell killing events after cell contact. K562 cells are indicated with green fluorescence and NK cells with yellow arrows. (B) Time for target cell killing (N=11) and (C) killing capacity of NK cells as determined from time-lapse images (N=12). (D) Killing capacity of NK cells cultured in AI or AI + 10% human serum (N=4) at 30, 90, 150, and 240 minutes. *P<0.05; **P<0.01; ***P<0.001.

Abbreviations: DS, DMEM+Ham’s F12+various supplements; AI, AIM V+ICSR; NK, natural killer; UCB, umbilical cord blood; ICSR, Immune Cell Serum Replacement; HM serum, human serum.