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. 2022 Jul 6;11(7):bio059459. doi: 10.1242/bio.059459

Fig. 1.

Fig. 1.

Embryonic kidney explants (E11.5) graft with low efficiency. (A) Kidney explants that appeared as a white disc were considered ungrafted. Scale bar: 500 µm. (B) Successfully grafted kidneys display visible size increase and blood vessels entering the kidneys. Scale bar: 500 µm. (C) Bar chart and scatter plot displaying difference in diameter between successfully grafted and ungrafted kidneys. Error bars display standard deviation. ***P<0.001 according to Student's t-test. (D) Induction of Vegf expression does not increase grafting efficiency. (E) Vegf expression after treatment with indicated doses of progesterone (P4), N=6. (F) Vegf expression after treatment with IGF-1, N=6. Treatment of kidney explants with P4 and IGF-1 increased Vegf expression by 50%. *P<0.05, Student's t-test, N=6. (G) Treatment with P4 and IGF-1 did not increase the grafting efficiency. ns, not significant. Scatter plot shows individual data points. The bar chart displays the average grafting efficiency across four experiments. During each experiment, four to 16 kidneys were grafted per treatment group. Error bars display standard deviation. Calculation of the 95% confidence interval indicated no statistical significance.