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. 2022 Jun 29;12:935806. doi: 10.3389/fcimb.2022.935806

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Copyright © 2022 Groeger, Wu, Wagenlehner, Dansranjav, Ruf, Denter and Meyle

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PD-L1 expression is up-regulated by P. gingivalis peptidoglycan (PGN) but not LPS. (A) Western blot of PD-L1 expressed by DU145 cells after 24h stimulation P. gingivalis isolated PGN. (B) PD-L1 expression is up-regulated in DU145 cells after 24h stimulation P. gingivalis isolated PGN. (n>3) ( **= p < 0.01.), neg = non- stimulated cells, TM, total membrane, peptidoglycan = 10 μL isolated P. gingivalis peptidoglycan. (C) No up-regulated expression was detected after stimulation with P. gingivalis LPS or E. coli LPS. (n=3). Neg = non-stimulated cells, E. coli and P. gingivalis LPS stimulation in a dose-dependent manner. ** indicates corrected p < 0.01 compared to control.