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. 2022 Jun 29;12:913619. doi: 10.3389/fcimb.2022.913619

Figure 2.

Figure 2

Genome organization and phylogeny of Diplodia seriata chrysovirus 1 (DsCV1). (A) Schematic representation of the DsCV1 (an alphachrysovirus) genome identified in Diplodia seriata isolate L3. Four DsCV1 genomic segments (dsRNA1 to dsRNA4) encode RNA-directed RNA polymerase (RdRP; P1), capsid protein (CP; P2) and hypothetical proteins encoded by dsRNA3 and dsRNA4. The open reading frames are shown by boxes of different colors in this and subsequent figures. The RdRP domain (RT like super family, pfam00680) was detected in P1 (RdRP) and is represented by a red bar in this and subsequent figures. The complete sequences of all the dsRNA segments were determined, except for dsRNA3, whose 5’-terminal end remained undetermined. (B) Phylogenetic tree utilizing the RdRP amino acid sequences derived from DsCV1 and other selected alpha- and betachrysoviruses. Maximum likelihood trees were constructed using PhYML online version 3.0 with 1000 bootstrap replicates under the best-fit model (LG+G+I+F) in this and the subsequent figures, unless otherwise mentioned. Values lower than 500 were masked.