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. 2022 Jul 2;2022:5045873. doi: 10.1155/2022/5045873

Figure 4.

Figure 4

miR-29a targets HBP1 3′UTR. (a) miRDB of downstream target prediction (target score ≥ 98) (http://mirdb.org/). (b) RT-qPCR was carried out to measure the levels of candidate mRNAs in the context of miR-29a upregulation. (c) The protein level of HBP1 after miR-29a upregulation was determined by western blotting. (d) TargetScan of binding site prediction (http://www.targetscan.org/vert_80/). (e) The binding sites between miR-29a and HBP1 among species. (f) Luciferase reporter assays were conducted to verify the binding relationship of miR-29a and HBP1. (g) The relationship between miR-29a and HBP1 was tested by RNA pull-down assays. ∗∗p < 0.01.