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. 2022 Jun 29;13:912089. doi: 10.3389/fpls.2022.912089

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Copyright © 2022 Nargar, O’Hara, Mertin, Bent, Nauheimer, Simpson, Zimmer, Molloy and Clements.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic relationships within Pterostylis based on maximum likelihood analysis of 75 plastid genes (91,090 bp alignment) with IQ-TREE. Labels A, B and C refer to the three major clades within the genus, C1 and C2 represent the two main clades within clade C. Nodal support values >50 are given above branches (ultrafast bootstrap values from IQ-TREE analysis followed by bootstrap values from RAxML analysis). Tree insert shows phylogenetic position of Pterostylidinae in orchid phylogeny (see Figure 3).