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. 2022 May 29;113(7):2297–2310. doi: 10.1111/cas.15384

FIGURE 5.

FIGURE 5

HOXC‐AS2 binds to miR‐876‐5p and regulates HKDC1 expression. (A) MiRNAs targeting HKDC1 were predicted by the TargetScan database. (B) qRT‐PCR analysis of miR‐3176 and miR‐876‐5p expression in EC cells treated with NG and HG (n = 5). (C) qRT‐PCR analysis of miR‐876‐5p expression in tumor tissues from patients with EC presenting normal blood glucose levels (NG) and tumor tissues from patients with EC complicated with diabetes mellitus (DM; n = 10). (D) Space structure docking model of HKDC1 and miR‐876‐5p, as predicted by the HDOCK server. (E) Western blot analysis of HKDC1 expression in Ishikawa and Hec‐1‐B cells after miR‐876‐5p agomir (mimics) was introduced (n = 5). (F) EC cells were cotransfected with a luciferase reporter construct carrying wild‐type (WT) or MUT HKDC1 and miR‐876‐5p mimics or NC and the luciferase activities were detected (n = 5). All values are presented as the mean ± SD, * P < 0.05, ** P < 0.01, and *** P < 0.001. EC, endometrial cancer; NC, negative control; HG, high glucose; MUT, mutant; NG, normal glucose; WT, wild‐type