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. 2022 May 29;113(7):2297–2310. doi: 10.1111/cas.15384

FIGURE 7.

FIGURE 7

The HOXC‐AS2/miR‐876‐5p/HKDC1 axis mediates EC cell pyroptosis, proliferation, and migration in response to HG. (A) Si‐HOXC‐AS2‐2 had the highest silencing efficiency (n = 5). (B) The expression of HKDC1 in Ishikawa and Hec‐1‐B cells transfected with si‐HOXC‐AS2, miR‐876‐5p agomir, and miR‐876‐5p antagomir (n = 5). (C) Expression of pyroptosis‐related proteins, including NLRP3, Caspase1, and pro‐Caspase1 (n = 5). (D) PI staining was performed to detect pyroptosis in different groups. Scale bar = 50 μm. (E, F) CCK‐8 and transwell assays were conducted to detect the proliferation and migration of EC cells (n = 5). All values are presented as the means ± SD, * P < 0.05, ** P < 0.01, and *** P < 0.001. agomir, miR‐876‐5p mimics; antagomir, miR‐876‐5p inhibitor; EC, endometrial cancer; HG, high glucose; NC, negative control; NG, normal glucose; si‐HOXC‐AS2, small interfering RNA targeting HOXC‐AS2