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Cumulative frequency histograms (normalized) of the Brillouin shift values of all (a) living U87-MG cell (n = 11) and all (b) living U87-MG spheroid (n = 9) mappings, showing that there are different contributions fitted by Gaussian curves: the main contributions are at 5.3 GHz and 5.4 GHz, respectively. (c) Brillouin shift frequencies of the main contribution of the individual maps are significantly different (Mann–Whitney U-test; ***p < 0.001).
