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. 2022 Jun 29;13:895696. doi: 10.3389/fphar.2022.895696

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Copyright © 2022 You, Wu, Li, Sheng, Zhou and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The main bio-active components in the herb pair A–S suppressed LNCaP cell proliferation and migration through the PI3K/AKT/mTOR pathway. (A) LC–MS/MS chromatogram of the bio-active components in Astragalus. (B) The LC-MS/MS analysis of PESV in scorpion. (C) LC3 and DAPI immunofluorescence staining were performed to detect autophagy. (D) The LC3, Beclin1, and P62 expression were determined by western blot. (E) The protein expression of the PI3K/AKT/mTOR signaling pathway. (F) Cell activity was determined by the CCK8 assay. *p < 0.05 vs. control group, #p < 0.05 vs. Astragaloside IV or PESV groups.