FIG. 2.

Effect of ethanol concentration on the tolerance of L. monocytogenes to pH 3.0. Cells were grown to the stationary phase (approximately 3 × 10⁹ CFU ml⁻¹) and diluted 1:100 in challenge media at pH 3.0, and viability was assessed in the presence of 0% (●), 1.25% (▴), 2.5% (■), 5% (⧫), or 10% (▾) ethanol. The data are means, and the error bars indicate standard deviations for experiments performed in triplicate. The limit of detection was 250 CFU ml⁻¹.