Figure 3.

In the proGIP gene, the coding region of the biologically active peptide is more conserved, and missense variants here are more deleterious. (A) Aggregated mean CADD scores (abbreviation of Combined Annotation-Dependent Depletion, predicting variant deleteriousness) for individual pre-pro-GIP regions demonstrate that variants in the GIP sequence exhibit a significantly higher mean CADD score than those of the other peptide regions. (B) Comparison of aggregated mean conservation scores (CS) indicates stronger evolutionary conservation relative to the other regions of the GIP gene central lines indicates median. Statistical significance between samples was assessed using the Mann-Whitney U test.