Figure 1.

eCIRP downregulates GPX4 expression in macrophages. RAW 264.7 cells were stimulated with (A) 1 μg/mL of rmCIRP for 1, 4 and 20 h and (B) 0.01, 0.1 and 1 μg/mL of rmCIRP for 20 h. Cells were collected and GPX4 expression was determined by Western blotting. Representative western blot showing GPX4 expression was obtained from a single experiment. The experiments were performed at least 2-3 times, and all the data obtained were analyzed to create the bar diagrams. Data were expressed as mean ± SEM (n = 4-6 sample/group). The groups were compared by one-way ANOVA and SNK method (*p < 0.05 vs. PBS).