Figure 5.

GPX4 expression is reduced, and MDA levels are increased in the lungs after sepsis in WT mice, but not in CIRP^−/− mice. After 20 h of CLP or sham procedure, lung tissues were collected from WT and CIRP^−/− mice and subjected to (A) Western blotting to assess GPX4 expression, (B) MDA assay to assess lipid ROS, and (C) iron assay. Representative western blot showing GPX4 expression was obtained from a single experiment. The experiments were performed at least 2 times, and all the data obtained were analyzed to create the bar diagrams. Data were expressed as means ± SE (n = 5-8 mice/group). The groups were compared by one-way ANOVA (*p < 0.05 vs. WT sham; ^#p < 0.05 vs. WT CLP mice). (D, E) After 20 h of CLP or sham procedure, lung tissues were collected from WT and CIRP^−/− mice. (D) Representative images of H&E-stained lung tissue at original magnification ×200. (E) Lung injury score calculated at original magnification ×400. n = 5 high-powered fields/group. Data were expressed as means ± SEM. The groups were compared by one-way ANOVA and SNK method (*p < 0.05 vs. WT sham; ^#p < 0.05 vs. WT CLP mice).