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. 2022 Jun 29;13:864225. doi: 10.3389/fimmu.2022.864225

Figure 2.

Figure 2

Met below 100 μM enhances IFNγ production by CD8+ T cells upon TCR stimulation. Splenic CD8+ T cells were cultured with the indicated concentrations of Met for 72, 96, and 120 h. The resulting cells were stimulated with PMA and ionomycin for 6 h, followed by the staining of intracellular IFNγ. (A) A representative flow cytometry plot showing the IFNγ levels in the CD8+ T cells. (B–D) The graph represents the percentage of the IFNγ+ CD8+ T cells at (B) 72 h, (C) 96 h, and (D) 120 h. (E–G) The graph represents the MFI of the IFNγ+ CD8+ T cells at (E) 72 h, (F) 96 h, and (G) 120 h. The graphs represent Mean ± SEM of the results of three independent experiments. Statistical analysis was performed by unpaired, two-tailed Student’s t-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001).