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. 2022 Jun 29;13:875013. doi: 10.3389/fgene.2022.875013

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Copyright © 2022 Tseng, Yang, Sung, Ding, Hsu, Chu and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of the mutant CNNM2. (A) mDCT cells were transfected with pcDNA3.1.HA.CNNM2-wild type, pcDNA3.1.HA.CNNM2-R480L, pcDNA3.1.HA.CNNM2-R480L, pcDNA3.1.HA.CNNM2-V548M, and pcDNA3.1.HA.CNNM2-T568I expression constructs. Cells extracts were subjected to western blot analysis and probed with antibodies against CNNM2. Antibodies to α-actin and Hsp70 were for loading control of mDCT cells. (B) Results are representative of three independent experiments and show increased membrane expression of CNNM2-R480L and CNNM2-R480K.