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Magnesium efflux assay. mDCT cells transfected with the indicated constructs were loaded with Mg2+ green and then subjected to the Mg depletion condition. R480L had significantly reduced Mg2+ efflux than other mutants and wild type. In comparison with wild-type and mutant, a p-value was calculated. A p-value <0.05 is considered statistically significant. *p<0.05, **p < 0.01.
