TABLE 1.
Effect of pH on conidiation and fumonisin productiona
Mediumb | Strain | Final pHc | Conidia (105)d | Dry wt (g) | FB1 concn (μg/ml)e |
---|---|---|---|---|---|
DL | Wild type | 2.4 ± 0.10 | 16 ± 1.2 | 1.0 ± 0.05 | 15 ± 0.71 |
FT536 | 2.4 ± 0.11 | 14 ± 1.4 | 0.9 ± 0.11 | 5.7 ± 0.54 | |
BSAL3 | Wild type | 2.6 ± 0.10 | 82 ± 13 | 1.4 ± 0.02 | 14 ± 0.60 |
FT536 | 2.7 ± 0.06 | 57 ± 16 | 1.1 ± 0.05 | 8.7 ± 0.98 | |
BSAL6 | Wild type | 5.6 ± 0.15 | 230 ± 14 | 1.8 ± 0.05 | 3.9 ± 0.91 |
FT536 | 5.6 ± 0.16 | 1.8 ± 0.22 | 1.8 ± 0.05 | NDf | |
BSAL9 | Wild type | 6.8 ± 0.05 | 240 ± 9.8 | 1.9 ± 0.05 | ND |
FT536 | 6.5 ± 0.05 | 1.9 ± 0.11 | 1.5 ± 0.05 | ND |
All data are means and standard deviations from three replicates.
DL, defined liquid medium (pH 5.9); BSAL, defined liquid medium with BSA as the sole nitrogen source (numbers indicate the initial pH of the medium).
Final pH of the fungal cultures after 7 days of incubation.
Determined with a hemacytometer.
Analyzed by HPLC.
ND, none detected.