Figure 4.

Analysis of neutrophil and eosinophil clusters of peripheral blood by cytometry in WT and IL-17RA-/- mice during T. canis infection. (A) Bar show frequency of total eosinophils (left) and neutrophils (right); (B) Expression levels of SiglecF; (C) Unsupervised high dimensional analysis of flow cytometry data (tSNE), gated on SiglecF+ eosinophils and SiglecF- and Ly6G+ neutrophils from combined data of blood obtained from WT 0dpi (red), WT 3dpi (green), IL17RA-/- 0dpi (blue) and IL17RA-/- 3dpi (purple); (D) tSNE of “C” representing cluster analysis by rPhenograph, eosinophils “E” and neutrophils “N”; (E) tSNE of “C” depicting neutrophil and eosinophil clusters per group; (F) Heat Map with cluster marker expression. Statistical analyses were performed between each strain with its uninfected group (0dpi), represented by the asterisk without the bar, and between the two strains at the same time of infection, represented by the asterisk with the bar. Non-significant differences were not reported in the bar. Data represented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001. According to the normality test, One-way ANOVA test followed by the Tukey test was used. N = 6 animals per group with two independent experiments.