Figure 7.

Flow Cytometry of bronchoalveolar lavage (BAL) analysis of leukocyte populations, alveolar macrophages, eosinophils, CD4+ and neutrophils in WT and IL-17RA-/- mice during T. canis infection. (A) The bar shows the frequency of alveolar macrophage; (B) The bar shows the mean intensity of fluorescence (MFI) of MHCII in alveolar macrophage; (C) The bar shows the frequency of IL-4 in alveolar macrophage; (D) The bar shows the frequency of IL-10 in alveolar macrophage; (E) The bar shows the frequency of CD4 T lymphocytes; (F) The bar shows the frequency of IL-17 in CD4 T lymphocytes; (G) The bar shows the frequency of eosinophils; (H) The bar shows the frequency of IL-10 in eosinophils; (I) The bar shows the MFI of MHCII in eosinophils; (J) The bar shows the frequency of IL-4 in eosinophils. ND= not-detected; (K) The bar shows the frequency of neutrophils; (L) The bar shows the frequency of MHCII in neutrophils; (M) The bar shows the frequency of IL-4 in neutrophils; (N) The bar shows the frequency of IL-10 in neutrophils; (O) The bar shows the frequency of IL-17 in neutrophils; (P) tSNE representing cluster analysis by rPhenograph, eosinophils “E”, neutrophils “N” and CD4 T lymphocytes “CD4”; (Q) tSNE of “P” depicting eosinophils, neutrophils and CD4 T lymphocytes clusters per group; (R) Heat Map with neutrophils cluster marker expression. Statistical analyses were performed between each strain with its uninfected group (0dpi), represented by the asterisk without the bar, and between the two strains at the same time of infection, represented by the asterisk with the bar. Non-significant differences were not reported in the bar. Data represented as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. For data that passed the normality test (A–E, G, K–N) One-way ANOVA test followed by the Tukey test was used, while non-parametric data (F, H, I, J, O) were used Kruskal-Wallis test followed by the Dunn test. N = 6 animals per group with two independent experiments.