. Author manuscript; available in PMC: 2022 Jul 13.

Published in final edited form as: Methods Mol Biol. 2021;2327:139–160. doi: 10.1007/978-1-0716-1518-8_9

Table 3.

Truncated example of PICRUSt2 output predicted absolute gene abundances using KEGG Orthologs (KOs) conducted on 16S rRNA sequencing data, with KOs in rows and Subject IDs in columns.

KEGG_orthology	ID1	ID2	ID3

K00367	34	205	29
K00370	10141	25010	31940
K00371	10388	7487	10450
...

Variable Key: Column heads have ID numbers, referring to the unique participant ID. Each row has one KEGG Ortholog (KO), defined as functional orthologs containing groups of genes. Cells contain absolute counts of that KO in the participant’s samples. Relative gene/KO abundances will be calculated before summing into a summary score.