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. 2022 Jul 12;12:107. doi: 10.1186/s13578-022-00840-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Reads distribution in the genome and natural species distribution of identified genes. A All the reads obtained were mapped to the cotton genome. Reads distribution in exon, intergenic, and intron regions were represented for ovules treatment with mock and TSA. B A total of 77,555 unigenes were annotated to the Nr protein database. For the species distribution, about 31,720 (47.92%), 17,814 (23%), 11,715 (15.12%), and 9030 (11.66%) unigenes were matched with Gossypium hirsutum, G. ramondii, G. barbadense and G. arboretum. The matched genes are less than 2% in other close species (i.e. Durio zibethinus, Theobroma cacao, Corchorus olitorius, Herrania umbratica)